Table 2.
List of conventional SARS CoV-2 RT-PCR kits.
| Manufacturer Details | Country of manufacturer | Regulatory status | Target Gene | Single tube multiplex/Multitube multiplex | PCR time | Instrument Compatibility | Pack Size | Reporter dye |
|---|---|---|---|---|---|---|---|---|
| International Manufacturer | ||||||||
| Altona Diagnostics | Germany | USA FDA EUA; CE-IVD | E gene, S gene with IC | SINGLE | 2.15 h | ABI 7500, Roche LightCycler® 480, RGQ, QuantStudio 5 R, CFX96, Mx 3005P™ QPCR System, L |
384 Rxn | FAM, JOE, Cy5 |
| Seegene | South Korea | USA FDA EUA; CE-IVD; Korea; Singapore, Australia; Canada |
E gene, RdRP gene, N gene with IC | SINGLE | 1.30 h | CFX 96 (BioRad) | 50/100 Rxn | FAM, CalRed 610, Quasar 670, HEX |
| SD Biosensor | Republic of Korea | USA EUA; CE-IVD; Korea; Brazil, CDSCO India |
ORF1ab,E gene with IC | SINGLE | 1.30 h | LightCycler 480 or CFX96Dx System, ABI 7500 | 96 Rxn | FAM, JOE/VIC/HEX, Cy5 |
| BGI | China | USA EUA; CE-IVD; China; Singapore; Canada |
ORF1ab region, β-Actin | SINGLE | 3 h | ABI 7500,Roche LightCycler 480,QuantStudio 5 | 50 Rxn | FAM/VIC (NOTspecified) |
| ABI (Applied bio systems) | USA | No web information | ORF1ab,S,N with IC | SINGLE | 1.10hrs | ABI 7500,QuantStudio 5 | 96 Rxn | FAM, VIC, ABY, JUN |
| ADT Biotech SdnBhd | Malaysia | RUO | E gene, RdRP gene with IC | SINGLE | 2.15 h | Rotor-Gene 3000/6000,Rotor-Gene Q5/6 plexPlatform,ABI 7500,CFx96 | 48 Rxn | FAM,HEX |
| OSANG Health Care | Republic of Korea | FDA EUA,CE-IVD | E, RdRP, N with IC | SINGLE (Single gene) | 2.15 h | CFX96Dx System, ABI 7500 | 100 Rxn | FAM,TexasRed,JOE/VIC,Cy5 |
| Gene Matrix | California, USA | USA FDA EUA | N, RdRP | SINGLE | 3 h | CFX96Dx, ABI 7500 | 96 Rxn | FAM, HEX |
| Accelerate Technologies Pte Ltd | Singapore | RUO | Not Specified | SINGLE | 2.30 h | CFX96Dx | 200 Rxn | FAM, HEX |
| Daan Gene Co. Ltd., | China | CE-IVD; China | ORF1ab, N | SINGLE | 1.30 h | LightCycler480 Ⅱ, ABI 7500 | 24/48/96 Rxn | FAM,VIC,Cy5 |
| JN MedsysPte Ltd, | Singapore | USA EUA, CE IVD Philippines | N1, N2 and RNase P | SINGLE | 1.35 Hrs | QS3 | 100 RX | FAM |
| Kogene Biotech | Korea | CE-IVD | E, RdRP | TWO | No web information | CFX 96, ABI 7500, PowerAmp 96 | 50/100Rxn | FAM, JOE (VIC/HEX) |
| LabGenomics, | South Korea | USA FDA EUA | E, RdRP | TWO | 2.30Hrs | CFX 96, ABI 7500 | 100 RX | :FAM and HEX (VIC), Cy5 |
| OSANG Health Care | South Korea | CE IVD, USA FDA EUA | E, RdRP, N | SINGLE | 2 Hrs | CFX 96, ABI 7501 | 100 RX | FAM,Texas Red, JOE/VIC,Cy5 |
| Primer Design, | UK | CE IVD/RUO∗ | N, RdRP | SINGLE | No web information | Roche LightCycler 480 II,CFX 96, ABI 7500 | 96 Rxn | FAM, VIC/HEX |
| Sansure Biotech Inc., | China | CE IVD, USA FDA EUA | ORF1ab, N | SINGLE | 2.30 Hrs | ABI 7500 | 24/48 Rxn | FAM, ROX, Cy5 |
| ZyBioInc, | China | USA FDA EUA | No web information | No web information | No web information | No web information | 32 Rxn | No web information |
| National Manufacturers | ||||||||
| MY LAB | India | DGCI India | RdRp, E gene RNAseP as IC |
THREE | 2 h | ABI 7500, RGQ, QS 5 R, CFX instrument (Bio-Rad) | 50 and 100 Rxn | FAM (RdRP) VIC/HEX (E and RNAseP) |
| KILPEST (BLACKBIO) | India | DGCI India | E, RdRP, N with IC | SINGLE/TWO | 1.10hrs | ABI 7500, RGQ, QuantStudio 3 | 100 Rxn | FAM, HEX |
| Helini Biomolecules | India | CE IVD, ICMR | RdRp and ORF gene | SINGLE | 1.35 Hrs | Agilent, Bio-Rad, Roche Lightcycler-96, RocheZ480/Cobas-480, ABI 7500, Thermo-Piko-Real, Rotor gene 5/6plex, Alta-96, Cepheid Real time PCR machines |
25/50/100 Rxn | FAM & HEX & Cy5 |
| GCC Biotech | India | ICMR | RdRP gene, N gene with IC | SINGLE | 1.35 Hrs | RGQ,QuantStudio 3 | 100/200/500 Rxn | No web information |