Table 3.
Isothermal DNA Amplification Technologies compared to PCR (a non isothermal method).
| Property | PCR | NASBA | SMART | SDA | RCA | LAMP | HDA | SPLA |
|---|---|---|---|---|---|---|---|---|
| DNA amplification | + | + | + | + | + | + | + | + |
| RNA amplification | + | + | + | + | + | + | + | + |
| Temperature in degree Centigrade (Celsius) | 94, 55–60, 72 | 37–42 | 41 | 37 | 37 | 60–65 | Room temp, 37, 60-65 | 45,50 |
| Primer design | Simple | Simple | Complex | Complex | Simple | Complex | Simple | Simple |
| Number of enzyme | 1 | 2–3 | 2–3 | 2 | 1 | 1 | 2 | 3 |
| Multiplex amplification | + | + | – | – | – | – | + | – |
| Product detection | Gel electrophoresis, Real-time | Gel electrophoresis, Real-time, ECL | Gel electrophoresis, Real-time, | Gel electrophoresis, Real-time, | Gel electrophoresis, Real-time, | Gel electrophoresis, Real-time, turbidity | Gel electrophoresis, Real-time, ELISA | Bioanalyzer |
• PCR: polymerase chain reaction; NASBA-nucleic acid sequence based amplification; SMART- Simple Method to Amplify RNA Targets; SDA-strand displacement amplification; RCA-recombinase polymerase amplification; LAMP- loop mediated isothermal amplification; HDA- Helicase Dependent Amplification; SPLA - Single-primer-limited amplification.