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. 2021 May 25;9:621906. doi: 10.3389/fcell.2021.621906

FIGURE 5.

FIGURE 5

Sp1 suppression prevented cardiomyocytes from OGD/R through PARP1 inhibition. The OGD/R-modeled H9c2 cells were treated with Sp1 shRNA-NC, Sp1 shRNA, PARP1 inhibitor AG-14361 and AD-PARP1 adenovirus. (A) Flow cytometry analysis showed that the percentages of necrotic and apoptotic cells when treating H9c2 cells with Sp1 shRNA, Sp1 shRNA-NC and AG-14361. (B) MTT assay was performed to analyze the cell viability of H9c2 cells when treating H9c2 cells with Sp1 shRNA, Sp1 shRNA-NC and AG-14361. (C) Immunofluorescence showed the positive rate of Sp1 when treating H9c2 cells with Sp1 shRNA, Sp1 shRNA-NC and AG-14361. Magnification: 200×. (D) Western blot showed the expression of PARP1 and autophagy related proteins when treating H9c2 cells with Sp1 shRNA, Sp1 shRNA-NC and AD-PARP1 adenovirus. (E) Real-time PCR showed the mRNA level of PARP1 and autophagy related proteins when treating H9c2 cells with Sp1 shRNA, Sp1 shRNA-NC and AD-PARP1 adenovirus. Compared with control group, *P < 0.05, **P < 0.01; compared with OGD/R group, #P < 0.05, ##P < 0.01; compared with OGD/R+AG-14361 group, $P < 0.05, $$P < 0.01. N = 4 for each group.