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. 2021 Jun 9;11:12178. doi: 10.1038/s41598-021-90982-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Differential gene enrichment analysis. (A) The difference between the differential genes in the HT22 group and the Purɑ-KO group is shown in the form of a heat map. (B) Histogram results of KEGG enrichment analysis of differential genes. The abscissa is the number of genes and the ordinate is the enrichment result. (C) GO enrichment of differential genes. The left aspect is the result of up-regulated gene enrichment and the right represents down-regulation. *RNA polymerase II sequence-specific DNA-binding transcription factor binding. **oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutrate as one donor, and incorporation of one atom each of oxygen into both donors.