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. 2021 Jun 8;9(6):e002273. doi: 10.1136/jitc-2020-002273

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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TLS image and immune cell densities. A representative multiplex image of a TLS is shown in (A–E) colors and scare bar are indicated. (A) Five color mIFH staining, (B) CD20⁺ B-cell aggregate, (C) CD8⁺ T-cell aggregate, (D) Ki67⁺ cells, (E) PNAd⁺ vasculature, (F) histogram of median densities within TLS. IQRs are shown in red bars. Kruskal-Wallis p<0.01. mIFH, multiplex immunofluorescence; PNAd, peripheral node addressin; TLS, tertiary lymphoid structures.