. 2021 Jun 9;11:12525. doi: 10.1038/s41598-021-91030-8

Table 1.

Summary of reproductive data.

Metric	M. capitata	Montipora spp.
Eggs per bundle	12.3 ± 0.2875 N = 8; n = 156	8.7 ± 0.213 N = 12; n = 156
Sperm concentration per bundle (mL)	1.5 × 10⁶ cells/mL ± 1.2 × 10⁵ N = 8; n = 142	7.5 × 10⁵ cells/mL ± 5.1 × 10⁴ N = 12; n = 142
Egg volume	0.04076 mm³ ± 0.00033 N = 16; n = 564	0.03903 mm³ ± 0.00055 N = 14; n = 564
Sperm motility	2017: 42% ± 8.1% N = 11 Pooled; n = 11 2018-Initial: 47% ± 2.1% N = 9; n = 55 2018-1Hr: 38% ± 5.1% N = 8; n = 31 2019-Initial: 63% ± 3.01% N = 16; n = 23 2019-1Hr: 64% ± 3.8% N = 16; n = 23	2017: 58% ± 7.1% N = 9; n = 16 2018-Initial: 84% ± 0.96% N = 16; n = 137 2018-1Hr: 82% ± 1.16% N = 16; n = 136 2019-Initial: 74% ± 2.1% N = 14; n = 61 2019-1Hr: 71% ± 2.07% N = 14; n = 61
Sperm with high mitochondrial membrane potential	2018: 36% ± 2.46% N = 9; n = 55 2019: 65% ± 1.86% N = 16; n = 23	2018: 78% ± 1.37% N = 16; n = 50 2019: 81% ± 1.2% N = 14; n = 61
Correlation of sperm motility and high membrane potential	2018: R² = 0.1025; p = 0.0172 N = 9; n = 55 2019: R² = 0.3715; p = 0.002 N = 16; n = 23	2018: R² = 0.2160; p < 0.0001 N = 16; n = 137 2019: R² = 0.3655; p < 0.0001 N = 14; n = 61
Fertilization	2018: 51% ± 5.71% N = 12; n = 14 2019: 20% ± 4.1% N = 19; n = 19	2018: 56% ± 5.09% N = 13; n = 18 2019: 60% ± 3.56% N = 26; n = 30
Settlement	2018: 7% ± 1.45% N = 7; n = 36 2019: 23% ± 4.92% N = 19; n = 18	2018: 16% ± 2.31% N = 19; n = 56 2019: 11% ± 1.81% N = 24; n = 55

Metric

M. capitata

Montipora spp.

Eggs per bundle

12.3 ± 0.2875

N = 8; n = 156

8.7 ± 0.213

N = 12; n = 156

Sperm concentration per bundle (mL)

1.5 × 10⁶ cells/mL ± 1.2 × 10⁵

N = 8; n = 142

7.5 × 10⁵ cells/mL ± 5.1 × 10⁴

N = 12; n = 142

Egg volume

0.04076 mm³ ± 0.00033

N = 16; n = 564

0.03903 mm³ ± 0.00055

N = 14; n = 564

Sperm motility

2017: 42% ± 8.1%

N = 11 Pooled; n = 11

2018-Initial: 47% ± 2.1%

N = 9; n = 55

2018-1Hr: 38% ± 5.1%

N = 8; n = 31

2019-Initial: 63% ± 3.01%

N = 16; n = 23

2019-1Hr: 64% ± 3.8%

N = 16; n = 23

2017: 58% ± 7.1%

N = 9; n = 16

2018-Initial: 84% ± 0.96%

N = 16; n = 137

2018-1Hr: 82% ± 1.16%

N = 16; n = 136

2019-Initial: 74% ± 2.1%

N = 14; n = 61

2019-1Hr: 71% ± 2.07%

N = 14; n = 61

Sperm with high mitochondrial membrane potential

2018: 36% ± 2.46%

N = 9; n = 55

2019: 65% ± 1.86%

N = 16; n = 23

2018: 78% ± 1.37%

N = 16; n = 50

2019: 81% ± 1.2%

N = 14; n = 61

Correlation of sperm motility and high membrane potential

2018: R² = 0.1025; p = 0.0172

N = 9; n = 55

2019: R² = 0.3715; p = 0.002

N = 16; n = 23

2018: R² = 0.2160; p < 0.0001

N = 16; n = 137

2019: R² = 0.3655; p < 0.0001

N = 14; n = 61

Fertilization

2018: 51% ± 5.71%

N = 12; n = 14

2019: 20% ± 4.1%

N = 19; n = 19

2018: 56% ± 5.09%

N = 13; n = 18

2019: 60% ± 3.56%

N = 26; n = 30

Settlement

2018: 7% ± 1.45%

N = 7; n = 36

2019: 23% ± 4.92%

N = 19; n = 18

2018: 16% ± 2.31%

N = 19; n = 56

2019: 11% ± 1.81%

N = 24; n = 55

Reproductive metrics assessed for Montipora capitata and Montipora spp. in 2018 and 2019 with mean and SEM. Number of unique genotypes (N), unique crosses (N), and sample replicates (n) used per analysis and year are given. Unique crosses are genotype combinations used for fertilization and settlement only. The coefficient of determination (R²) is given for the proportion of variance in percent sperm motility explained by the variation in percent of sperm with high mitochondrial membrane integrity.