Fig. 6. Time-dependent proliferation of TH+ cells in CRISPR/Cas9 MPS-IIIA cell line.

a SH-SY5Y MPS-IIIA showed increased cell density at DIV4, compared to WT controls, but not at DIV1. Representative staining for each condition are reported (scale bar: 10 µm). b Similarly, while at DIV1 there was no difference in the DA release, at DIV4 MPS-IIIA cells increased DA release. c MTS, and d BrdU assays confirmed that SH-SY5Y MPS-IIIA cells show an increased proliferation rate, compared to WT cells at DIV4 but not at DIV1. Histograms represent mean ± S.E.M. ^§p < 0.05 vs DIV1, within groups; ^*p < 0.05 vs WT, between groups.