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. 2021 Jun 9;12(6):597. doi: 10.1038/s41419-021-03866-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A circUBA1 originates from back-spliced exons 1 to 2 of C16orf62. The back-splice junction of circC16orf62 was identified by Sanger sequencing. B PCR analysis for circC16orf62 and linear C16orf62 in cDNA and gDNA. C The stability of circC16orf62 was evaluated by RNase R digestion assay. D PCR analysis for circC16orf62 and linear C16orf62 in cDNA and gDNA by RNase R digestion assay. E Nucleoplasmic separation analysis the location of circC16orf62. F FISH assays showed that circC16orf62 was distributed principally in the cytoplasm, the scale bar represents 10 µm. Data are shown as the mean ± standard deviation of three independent experiments. *P < 0.05was regarded as statistically significant.