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. 2021 Jun 9;12(6):597. doi: 10.1038/s41419-021-03866-7

Fig. 3. circC16orf62 promotes the proliferation, metastasis, and glycolysis of HCC cells.

Fig. 3

A The effects of knockdown or overexpression of circC16orf62 in Hep3B or SMMC7721 cells were measured using qRT-PCR. B–E CCK-8 and colony formation assays of PC cells with circC16orf62 knockdown. F, G Transwell assays showed that invasion and migration were significantly inhibited after circC16orf62 knockdown, the scale bar represents 50 µm. H–K Glucose consumption (H), lactate production (I), pyruvate production (J), ATP production (K) were measured in circC16orf62 knockdown HCC cells. L, M RT-qPCR and western blot analysis several metabolic enzymes (GLUT1, PKM2, HK2) expression level were in circC16orf62 knockdown HCC cells. Data are shown as the mean ± standard deviation of three independent experiments. *P < 0.05 was regarded as statistically significant.