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. 2021 Jun 9;11:12161. doi: 10.1038/s41598-021-91598-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of DEGs enrichment in flavonoid biosynthesis (Ko00941, cited from Kanehisa laboratories) in G vs CK seeds (A), genes marked with red indicate up-regulated, yellow has both up- and down-regulated genes in germinated seeds. The expression of flavonoid biosynthesis pathway genes by qPCR determination (B), expression levels estimated using log2(fold change) for each transcript (**p < 0.01). In the histogram, CK_q and G_q represents qPCR analysis; CK and G represent the FPKM value of RNA_seq.