Table 1.
Features of the assays used for semiquantitative real time polymerase chain reaction (qRT-PCR) for validation and conformation of microarray measurements.
| Gene symbol | Accession number | Assay ID | Assay location | Amplicon length | |
|---|---|---|---|---|---|
| Reference genes | |||||
| Rattus norvegicus beta-2 microglobulin | B2m | NM_012512 | Rn00560865_m1 | 86 | 58 |
| Rattus norvegicus actin, beta | Actb | NM_031144 | Rn00667869_m1 | 881 | 91 |
| Rattus norvegicus glyceraldehyde-3-phosphate dehydrogenase | Gapdh | NM_017008 | Rn01775763_g1 | 1153 | 174 |
| Rattus norvegicus hypoxanthine phosphoribosyltransferase 1 | Hprt1 | NM_012583 | Rn01527840_m1 | 673 | 64 |
| Target genes | |||||
| Rattus norvegicus gap junction protein alpha 5 | Gja5 | NM_019280 | Rn00570632_m1 | 60 | 83 |
| Rattus norvegicus solute carrier family 2 member 1 | Slc2a1 | NM_138827.1 | Rn01417099_m1 | 1281 | 73 |
| Rattus norvegicus solute carrier family 7 member 1 | Slc7a1 | NM_138827.1 | Rn00565399_m1 | 961 | 76 |
| Rattus norvegicus solute carrier family 38, member 5 | Slc38a5 | NM_138854.1 | Rn00684896_m1 | 610 | 59 |
| Rattus norvegicus ATP binding cassette subfamily C member 4 | Abcc4 | NM_133411.1 | Rn01465702_m1 | 914 | 64 |
| Rattus norvegicus platelet derived growth factor receptor beta | Pdgfrb | NM_031525 | Rn00709573_m1 | 726 | 53 |