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. 2021 Jun 9;11:12225. doi: 10.1038/s41598-021-90833-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Long-term co-culture viability is high in PREDICT96 devices. Representative co-culture devices assessed for viability at (A) day 7 and (B) day 14 with split channels in descending order: red, green, blue, overlay. (C) Representative co-culture device stained for actin fibers (Phalloidin) and ECs (PECAM-1) on day 14 shows good coverage for both cell types. (D) Viability was greater than 95% at days 7 and 14. (E) Total cell numbers as determined by nuclear counts in channel overlap at day 7 and 14 did not differ significantly. N = 2 at day 7; N = 4 at day 14.