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. 2021 Jun 9;11:12225. doi: 10.1038/s41598-021-90833-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Permeability assays in PREDICT96 vascular models. FITC-dextran was loaded at a concentration of 50 μg/ml into the top channel of devices and transfer to the bottom channel was tracked over time. Note that equilibrium between both channels would be at 25 μg/mL (indicated by black dotted line). Both EC mono-culture (Mono) and co-culture with PC (Co) were assessed. Treatment with cytochalasin B (CB) was used to disrupt the barrier (added at t = 0). Transfer of (A) 20 kDa FITC-dextran and (B) 70 kDa FITC-dextran across the vascular barrier was measured at various intervals over 260 min. (C) Permeability coefficients were calculated at the 260 min time point for each condition assayed in the PREDICT96 plate. N = 8–10 technical replicates per condition. Asterisks in A and B indicate significant difference in + CB treated conditions compared to -CB controls at corresponding time points.