Fig. 6.

The effects of STAMBP are blocked by small molecule inhibitors of EGFR/MAPK signaling. (A-B) H1299 and H-1 cells were treated with or without 100 ng/ml EGF for 48 or 72 h. The cells were treated with or without the EGFR inhibitor AG1478 (4 μM), the RAS inhibitor salirasib (140 μM), the RAF inhibitors TAK580 (10 μM) and LY3009120 (10 μM), the MEK inhibitor U0126 (10 μM), the ERK inhibitor LY3214996 (10 μM), and the PI3K inhibitor LY294002 (5 μM). The migratory and invading cells on the underside of the filter were fixed, stained and counted. The experiments were repeated twice, and a representative result was shown. (C) H1299 and H1 cells were treated as indicated above for 24 h. Western blot analysis of cell extracts was performed with indicated antibodies.