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. 2021 Apr 24;25:37–52. doi: 10.1016/j.omtn.2021.04.016

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

PRR34-AS1 promotes the progression of HCC via upregulating E2F2 and SOX12

(A and B) Quantitative real-time RT-PCR and western blot assay examined the expression of E2F2 and SOX12 in HCCLM3 cells under diverse transfections. (C and D) CCK-8 and colony formation experiments assessed the proliferation of indicated HCCLM3 cells. (E and F) TUNEL assays and flow cytometry analysis detected HCCLM3 cell apoptosis rate in different groups. (G–I) Wound healing and Transwell assays assessed the migration and invasion capacities of indicated HCCLM3 cells. (J) Western blot assay analyzed the protein levels of E-cadherin, N-cadherin, and Vimentin in HCCLM3 cells with diverse transfections. ∗p < 0.05.