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. 2021 Apr 29;21:367–376. doi: 10.1016/j.omto.2021.04.011

Figure 2.

Figure 2

E2F1 augments the viability, migration, and invasion of NSCLC cells

(A) Western blot to determine the silencing effect of plasmids carrying different E2F1-targeting shRNAs in NSCLC cells. (B) Western blot to determine the overexpression effect of different E2F1 overexpression plasmids in NSCLC cells. (C) CCK-8 assay to detect A549 cell viability in response to overexpression or silencing of E2F1. (D) EdU staining to detect A549 cell viability in response to overexpression or silencing of E2F1. (E) Wound-healing assay to detect the migration of A549 cells in response to overexpression or silencing of E2F1. (F) Transwell assay to evaluate A549 cell invasion in response to overexpression or silencing of E2F1. ∗p < 0.05. One-way ANOVA was applied for comparison among multiple datasets and two-way ANOVA for comparison among data of multiple datasets at different time points. Each experiment was conducted in triplicates.