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. 2021 Apr 29;21:367–376. doi: 10.1016/j.omto.2021.04.011

Figure 5.

Figure 5

The FTO/E2F1/NELL2 axis modulates NSCLC cell viability, migration, and invasion

(A) qRT-PCR to determine the expression of FTO, E2F1, and NELL2 in A549 cells in response to FTO overexpression alone or its combination with E2F1/NELL2 knockdown. (B) CCK-8 assay to detect A549 cell viability in response to FTO overexpression alone or its combination with E2F1/NELL2 knockdown. (C) EdU staining to detect A549 cell viability in response to FTO overexpression alone or its combination with E2F1/NELL2 knockdown. (D) Wound-healing assay to detect A549 cell migration in response to FTO overexpression alone or its combination with E2F1/NELL2 knockdown. (E) Transwell assay to detect A549 cell invasion in response to FTO overexpression alone or its combination with E2F1/NELL2 knockdown. ∗p < 0.05. One-way ANOVA was applied for comparison among multiple datasets and two-way ANOVA for comparison among data of multiple datasets at different time points. Each experiment was conducted in triplicates.