Fig. 1.

Effects of age and anti-CD3 treatment on tissue T cell infiltration. The spleen (a and b), epididymal white adipose tissue (eWAT) (c and d), subcutaneous white adipose (scWAT) (e and f), brown adipose (BAT) (g and h), liver (i and j), and quadriceps (k and l) were dissected from young and old, isotype control and anti-CD3 F(ab’)2-treated mice. Tissues were mechanically disrupted, enzymatically digested, and stained with anti-CD45 (leukocytes), and anti-CD3 (T cells) dead cells were excluded with violetFluor510 Ghost Dye. Group differences were assessed by two-way ANOVA and Tukey’s post hoc test