Fig. 6.

Effects of age on Treg proportion and accumulation in the eWAT and liver. Epididymal white adipose tissue (eWAT) and livers from young and old mice were dissected, mechanically disrupted, enzymatically digested activated in vitro, and stained for CD45 (total leukocytes), CD3 (pan T cells), CD4, CD8, CD25, and Foxp3. CD25+, Foxp3+ cells are considered Tregs. a Treg proportion and b accumulation in eWAT. c Treg proportion and d accumulation in liver. Group differences were assessed with an independent sample T test; p values are included on the panel with a line indicating the group comparison