Table 5.

Fertilization rates, blastocyst development rates, and clinical outcome by time from ICSI to ROA

	<4 h	≥4 h	P value
ROA oocytes	169	141
1PN rate, n (%)	23 (13.6%)	17 (12.1%)	0.46
2PN rate, n (%)	132 (78.1%)	118 (83.7%)
3PN or higher rate, n (%)	7 (4.1%)	4 (2.8%)
0PN rate, n (%)	7 (4.1%)	2 (1.4%)
Blastocyst development rate, n (% per 2PN)	67 (50.7%)	57 (48.3%)	0.98
Good quality blastocyst development rate, n (% per 2PN)	28 (21.2%)	23 (19.5%)	1.00
Single-blastocyst transfer number	15	10
implantation rate, n (% per blastocyst transfer)	6 (40.0%)	3 (30.0%)	0.93
Miscarriage rate, n (% per clinical pregnancy)	2 (33.3%)	2 (66.7%)	0.81
Live birth rate, n (% per blastocyst transfer)	4 (26.7%)	1 (10.0%)	0.61

ICSI intracytoplasmic sperm injection, ROA rescue artificial oocyte activation