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. 2021 Jun 9;12:3497. doi: 10.1038/s41467-021-23636-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic overview of 12-mer chromatosome array formation containing H1.2 or H1.2 KxUbs. b EMSA of different arrays analyzed; crDNA indicates competitor DNA. c Overview of MNase digestion assay which probes DNA accessibility of nucleosome and chromatosome arrays (left). The arrays were treated with MNase for indicated times, digested by proteinase K, and analyzed by agarose gel electrophoresis. Undigested arrays were quantified and plotted over time (middle). Shown are mean values +/− standard deviation, n = 3. For statistical analysis, the area under the curve for the digestion of the different arrays was determined and analyzed (right); shown are mean values +/− standard deviation, n = 3, one-way ANOVA with Tukey’s multiple comparisons test with α = 0.05, 95% confidence interval, exact p values with p ≤ 0.05 are shown, ns (not significant) p > 0.05. d PacI digestion assay. PacI cleaves the array site-specifically within the linker DNA between the repetitive nucleosome positioning sequences resulting in the formation of mono-nucleosomes and mono-chromatosomes.