Figure 1.

Stimulated Raman scattering histology (SRH) workflow for intraoperative tumor margin tissue assessment. Tumor samples obtained from the lesion core were divided for routine intraoperative evaluation using cytologic smear preparations and/or frozen section, and the remaining tissue was submitted for SRH imaging. Upon completion of resection, samples obtained from the resection cavity margins are submitted for SRH imaging. SRH images were obtained, and pseudo-H&E images were rendered. Tissues used for SRH are subsequently placed in 10% formaldehyde and submitted for histologic processing, paraffin embedding, sectioning, and staining. SRH images and histology sections from margins were retrospectively reviewed.