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. 2021 Jun 9;11:12162. doi: 10.1038/s41598-021-91648-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Heatmaps representing scores provided for each margin sample using Hematoxylin & Eosin (H&E) stained sections, Stimulated Raman Microscopy (SRH) images, and combined immunohistochemistry (IHC) for IDH1 R312H and p53. (A) Results of the 4-tier scoring system including consensus score and individual pathologist scores based on H&E, SRH, and IHC. (B) Results of the 2-tier, binary scoring system (tumor present vs. absent) including consensus score and individual pathologist scores based on H&E, SRH, and IHC. IHC score is not available for infiltrative gliomas which are IDH1 R132H-negative and p53-negative. Consensus score was rendered using the score provided by at least two pathologists for each modality for each margin. Consensus score was “not available” if pathologists provided three different scores. (C) Cellularity across different SRH consensus scores. There was a significant correlation between SRH consensus scores and cellularity of the tissue (p < 0.0001). Cell count (cell/mm²). (D–F) Samples with residual tumor demonstrated higher cellularity than samples without tumor for both low and high-grade gliomas, using a 2-tiered scoring system for all modalities. Panels (D–F) demonstrate the cellularity based on SRH, H&E and IHC (p < 0.0001).