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. 2021 Jun 9;12:3486. doi: 10.1038/s41467-021-23856-9

Fig. 4. dhfr-1 and 5MTHF act within insulin/IGF and mitochondrial signaling pathways.

Fig. 4

a Life span of daf-2(e1370) supplemented with 10 nM 5MTHF (from L4 stage). b Life span of isp-1(qm150) supplemented with 10 nM 5MTHF (from L4 stage). 5MTHF does not alter WT life span but reduces daf-2 and isp-1 longevity. c, d Relative mRNA expression of folate cycle genes in daf-2(e1370) and isp-1(qm150) (day 1 adult). dhfr-1 mRNA expression is lower in both genotypes. e Life span experiment of wild-type and daf-16(mgDf50) with luci and dhfr-1i in the presence or absence of 5MTHF (from L4 stage). dhfr-1i extends daf-16 mutant life span and supplementation with 5MTHF abolishes this extension. f Relative mRNA expression of folate cycle genes in daf-16 and daf-16,daf-2 backgrounds. daf-16 mutation has little or no effect on dhfr-1 or mthf-1 mRNA expression in the daf-2 background. a, b, e n = 150 per repeat per condition, N = 3 independent biological replicates. c, d, f N = 3 biological replicates. a, b, e Statistics were analyzed by the two-sided Mantel–Cox log-rank test (Supplementary Table 7 for statistics). c, d, f Significance was assessed using one-way ANOVA and Dunnett’s multiple comparisons test. *p < 0.5, **p < 0.01, ***p < 0.001. Data are presented as mean ± S.D. (Supplementary Table 6 for statistics).