Table 2.
Overexpressed pathways in differentially* expressed genes at 24 and 48 h in L. monocytogenes isolates 7453F and 7545F.
| Locus tag (EGD-e) | Locus tag (this study) | Fold change | Gene | COG cat | Description | KEGG enzyme | Isolate and TP# |
|---|---|---|---|---|---|---|---|
| PTS system | |||||||
| lmo1035 | fig|1639.4014.peg.1355 | 4.22 | G | PEP-dependent sugar PTS, EIIA 1 | 7453 24 h | ||
| lmo1719 | fig|1639.4014.peg.1473 | 4.36 | G | PTS system cellobiose-specific IIA component | 2.7.1.205 | ||
| lmo0427 | fig|1639.4014.peg.2115 | 4.77 | G | PTS system, Lactose/Cellobiose specific IIB subunit | |||
| lmo0426 | fig|1639.4014.peg.2116 | 4.02 | G | PEP-dependent sugar PTS, EIIA 2 | |||
| lmo0024 | fig|1639.4014.peg.434 | 4.99 | G | PTS system mannose/fructose/sorbose family IID component | |||
| lmo0034 | fig|1639.4014.peg.444 | 5.61 | G | PTS system cellobiose-specific IIC component | |||
| lmo0374 | fig|1639.4014.peg.610 | 3.66 | G | PTS system cellobiose-specific IIB component | 2.7.1.205 | ||
| lmo0874 | fig|1639.4014.peg.773 | 6.1 | G | PTS system, Lactose/Cellobiose specific IIA subunit; PTS system beta-glucoside-specific IIA component | |||
| lmo0503 | fig|1639.4014.peg.936 | 3.48 | G | PTS system galactitol-specific IIA component | 2.7.1.200 | ||
| Starch and sucrose metabolism | |||||||
| lmo0271 | fig|1639.4014.peg.1428 | 4.47 | G | Glycosyl hydrolase 1 family; 6-phospho-beta-glucosidase | 3.2.1.86 | 7453 24 h | |
| lmo1719 | fig|1639.4014.peg.1473 | 4.36 | G | PTS lichenan-specific enzyme IIA component; PTS system beta-glucoside-specific IIA component; PTS system cellobiose-specific IIA component | 2.7.1.205 | ||
| lmo2840 | fig|1639.4014.peg.393 | 4.82 | YcjM | G | Sucrose glucosyltransferase/sucrose phosphorylase (ycjM) | 2.4.1.7 | |
| lmo0034 | fig|1639.4014.peg.444 | 5.61 | G | PTS system cellobiose-specific IIC component | |||
| lmo0372 | fig|1639.4014.peg.608 | 4.16 | G | Glycosyl hydrolase 1 family; 6-phospho-beta-glucosidase | 3.2.1.86 | ||
| lmo0374 | fig|1639.4014.peg.610 | 3.66 | G | PTS system cellobiose-specific IIB component | 2.7.1.205 | ||
| lmo0874 | fig|1639.4014.peg.773 | 6.1 | G | PTS system, Lactose/Cellobiose specific IIA subunit; PTS system beta-glucoside-specific IIA component | |||
| lmo1729 | fig|1639.4024.peg.1538 | 3.27 | G | Glycosyl hydrolase 3 family; beta-glucosidase | 3.2.1.21 | 7545 24 h | |
| lmo0372 | fig|1639.4024.peg.670 | 3.69 | G | Glycosyl hydrolase 1 family; 6-phospho-beta-glucosidase | 3.2.1.86 | ||
| lmo2121 | fig|1639.4024.peg.985 | 2.87 | G | Trehalose and maltose hydrolases; Maltose phosphorylase | 2.4.1.8 | ||
| Cobalamin biosynthesis | |||||||
| lmo1148 | fig|1639.4014.peg.281 | 5.05 | cobS | H | Cobalamin synthase | 2.7.8.26 | 7453 24 h |
| lmo1192 | fig|1639.4014.peg.237 | 3.51 | cobD | H | Adenosylcobinamide-phosphate synthase | 6.3.1.10 | |
| lmo1194 | fig|1639.4014.peg.235 | 3.95 | cbiD | H | Cobalt-precorrin-5B (C1)-methyltransferase | 2.1.1.195 | |
| lmo1191 | fig|1639.4014.peg.238 | 3.35 | cbiA | H | Cobyrinic acid c-diamide synthetase | 6.3.5.11 | |
| lmo1204 | fig|1639.4014.peg.225 | 4.61 | cbiM | P | Cobalt ECF transporter substrate-binding protein CbiM | ||
| Prophage related genes | |||||||
| fig|1639.4014.peg.1757 | 3.84 | K | BRO family, N-terminal domain; Antirepressor [Bacteriophage A118] | 7453 24 h | |||
| fig|1639.4014.peg.1796 | 3.38 | N | Bacterial Ig-like domain 2; Protein gp13 [Bacteriophage A118] | ||||
| fig|1639.4014.peg.2230 | 3.41 | S | Phosphoadenosine phosphosulfate; Co-activator of prophage gene expression IbrA | ||||
| fig|1639.4014.peg.2484 | 5.39 | S | Phage protein | ||||
| fig|1639.4014.peg.1804 | 4.2 | S | Putative short tail fibre [Bacteriophage A118] | ||||
| fig|1639.4014.peg.1780 | 4.16 | S | Protein of unknown function (DUF2481) [Bacteriophage A118] | ||||
| fig|1639.4014.peg.2780 | 4.01 | S | Prophage endopeptidase tail | ||||
| fig|1639.4014.peg.1788 | 3.89 | S | Phage minor capsid protein 2 | ||||
| fig|1639.4014.peg.1793 | 3.87 | S | Minor capsid protein | ||||
| fig|1639.4014.peg.1805 | 3.8 | S | Protein gp22 [Bacteriophage A118] | ||||
| fig|1639.4014.peg.2062 | 3.61 | S | Phage tail tape measure protein | ||||
| fig|1639.4014.peg.2783 | 3.41 | S | COG5546 Small integral membrane protein | ||||
| fig|1639.4014.peg.1787 | 3.34 | S | Phage portal protein, SPP1 Gp6-like [Bacteriophage A118] | ||||
| fig|1639.4014.peg.1759 | 6.2 | S | Protein gp44 [Bacteriophage A118] | ||||
| fig|1639.4014.peg.468 | 5.43 | V | Type VII secretion protein EsaA | ||||
| Amino sugar and nucleotide sugar metabolism | |||||||
| lmo0957 | fig|1639.4014.peg.2911 | 3.89 | nagB | G | Glucosamine-6-phosphate deaminase | 3.5.99.6 | 7453 48 h |
| lmo0956 | fig|1639.4014.peg.2912 | 3.55 | nagA | G | N-acetylglucosamine-6-phosphate deacetylase | 3.5.1.25 | |
| lmo0096 | fig|1639.4014.peg.505 | 3.03 | G | PTS system mannost-specific transporter subunits IIAB | 2.7.1.191 | ||
| lmo0097 | fig|1639.4014.peg.506 | 2.99 | G | PTS system mannose-specific IIC component | |||
| Lmo0783 | fig|1639.4014.peg.1297 | 4.18 | manX | G | PTS system mannose-specific IIAB component | 2.7.1.191 | |
| lmo2552 | fig|1639.4014.peg.192 | 3.7 | murZ | M | UDP-N-acetylglucosamine 1-carboxyvinyltransferase | 2.5.1.7 | |
| Ribosome | |||||||
| lmo1542 | fig|1639.4024.peg.1293 | 3.82 | rplU | J | LSU ribosomal protein L21p | 7545 48 h | |
| lmo0250 | fig|1639.4024.peg.1620 | 3.36 | rplJ | J | LSU ribosomal protein L10p (P0) | ||
| lmo2629 | fig|1639.4024.peg.266 | 4.02 | rplB | J | LSU ribosomal protein L2p (L8e) | ||
| lmo2622 | fig|1639.4024.peg.273 | 3.85 | rplN | J | LSU ribosomal protein L14p (L23e) | ||
| lmo2618 | fig|1639.4024.peg.277 | 4.56 | rpsH | J | SSU ribosomal protein S8p (S15Ae) | ||
| lmo2617 | fig|1639.4024.peg.278 | 4.01 | rplF | J | LSU ribosomal protein L6p (L9e) | ||
| lmo2616 | fig|1639.4024.peg.279 | 3.85 | rplR | J | LSU ribosomal protein L18p (L5e) | ||
| lmo2613 | fig|1639.4024.peg.282 | 3.57 | rplO | J | LSU ribosomal protein L15p (L27Ae) | ||
| lmo0044 | fig|1639.4024.peg.808 | 4.46 | rpsF | J | SSU ribosomal protein S6p | ||
*FDR < 0.01 log2 fold change.
#Time point.