Table 1.

PCR Conditions Used for FAS and FASL RFLP Analysis

SNP Polymorphism	Primer sequences	Annealing temperature	Restriction Enzyme used	Product size
FAS-670 A>G (rs1800682) (10q23.31)	F 5’-CTACCTAAGAGCTATCTACCGTTC-3’ R 5’-GGCTGTCCATGTTGTGGCTGC-3’	61˚C for 30s	BstN1	A allele 231 and 101bp G allele 184,101 and 47bp
FASL-844 T>C (rs763110) (1q24.3)	F 5’-GAAGCTAGGAGTTTGAGACAAG-3’ R 5’-ACACAACTACCATTTACCCTGA-3’	64.4˚C for 30s	BSrD1	T allele 274 and 132bp C allele 406bp