Figure 1.

MCF-7 Cells Viability Analysis after Daidzein Treatment. MCF-7 cells were treated with varying concentrations of daidzein for 24, 48 and 72 h. After treatment, 20 μl of 5 mg/ml MTT was added and incubated for 4 hours at 37o C. After this, 100 µl of DMSO was added to each well to dissolve the resulting formazan crystals. Absorbance was measured at 490 nm in and ELISA reader. Data are presented as means of standard deviation from three independent experiments. Each experiment was conducted in triplicate (P = 0.047).