Figure 5.

Generation of Reactive Oxygen Species (ROS) in 50μm and 100 μm Daidzein Treated MCF-7 Cells. There is an outburst of ROS in 50 and 100 μm Daidzein treated cells as against control cells. As can be seen, the ROS emission is higher in 50 μm Daidzein treated cells in both the time frames, this is justified as the standard inhibitory concentration of daidzein which is capable of inducing apoptosis. Luminescence is higher in +ve control (0.03% H₂O₂). The data is statistically significant in all the four subsets of observation with p value in each case being 0.001