Table 3.
List of antibodies used in this study.
| Primary antibodies | |||||||
|---|---|---|---|---|---|---|---|
| Antigen | Host species | Dilution | Manufacturer | Catalog no. | Analysis | ||
| Iba-1 | Rabbit polyclonal | 1:500 | Wako | 019-19741 | IF | ||
| GFAP | Rabbit polyclonal | 1:500 | Neuromics | RA22101 | IF | ||
| IL-1β | Rabbit polyclonal | 1:200 | Abcam | AB9722 | IF | ||
| COX-2 | Rabbit polyclonal | 1:500 | Abcam | AB15191 | IF | ||
| p-AKTS473 | Rabbit polyclonal | 1:500 | Cell Signaling | 9271 | WB/IF | ||
| AKT | Rabbit polyclonal | 1:500 | Cell Signaling | 9272S | WB | ||
| p-STAT3S727 | Rabbit polyclonal | 1:500 | Abcam | AB86340 | ICC/IF | ||
| p-NF-κBS536 | Rabbit polyclonal | 1:500 | Cell Signaling | 3033S | ICC | ||
| p-P38T180/Y182 | Rabbit polyclonal | 1:1000 | Abcam | 9211 | WB | ||
| P38 | Rabbit polyclonal | 1:1000 | Abcam | 9212 | WB | ||
| CD11b | Rat monoclonal | 1:200 | Abcam | AB8878 | ICC | ||
| Secondary antibodies | |||||||
| Antibody | Dilution | Manufacturer | Catalog no. | Analysis | |||
| Goat anti-rabbit IgG, Alexa Fluor 488 | 1:200 | Invitrogen | A11008 | IF | |||
| Goat anti-rabbit IgG, Alexa Fluor 555 | 1:200 | Invitrogen | A28180 | IF, ICC | |||
| Goat anti-chicken IgG, Alexa Fluor 488 | 1:500 | Invitrogen | A11001 | IF | |||
| Goat anti-rat IgG, Alexa FITC | 1:200 | Invitrogen | A18866 | ICC | |||
| Goat anti-rabbit IgG, HRP conjugate | 1:10000 | Enzo | ADI-SAB-300-J | WB | |||
| Goat anti-mouse IgG, HRP conjugate | 1:10000 | Enzo | ADI-SAB-100-J | WB | |||