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. 2021 Jun 9;11:12157. doi: 10.1038/s41598-021-91231-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Examination of the presence of ACE2 in human ECs. (a) Expression of ACE2 in siMock and siACE2-transfected HPAECs. (b,c) Immunofluorescent staining of HPAECs transfected with ACE2-targeting (siACE2) and non-targeting (siMock) siRNA, with anti-ACE2 antibody. Representative photographs and quantitative analysis of immunofluorescence intensity. (d,e) Immunofluorescent staining of ACE2 in HPAECs and HBECs. Representative photographs and quantitative analysis of immunofluorescence intensity. Statistics: p values were determined by one-way ANOVA followed by Tukey’s post-hoc test. Figure created with OriginPro2021 (https://www.originlab.com/2021) and ImageJ 1.53e (https://imagej.nih.gov/ij/).