Fig. 1.

Elevated monocyte counts in PHD2^WT Tibetans than PHD2^D4E;C127S at high altitude. Monocyte count was measured in PBMCs of healthy Tibetans carrying either homozygous PHD2^D4E;C127S or PHD2^WT from high altitude and sea level using flow cytometry. (a) Percentage of monocytes in total leukocyte, (b) inflammatory and (c) patrolling subsets in total monocytes were measured. The detail immunophenotyping gating strategy is described in Fig. S1. Each dot represents the percentage of cells in each individual. Kruskal Wallis test was used on median values for comparison between genotypes followed by Dunn's multiple comparison post test, ***p<0.001, **p<0.01, *p<0.05 and ns=non-significant. Classical monocyte subtype count is mentioned in Fig. S2d. Also the monocyte count was analysed from whole blood using haematology analyser; these data show similar trend, depicted in Fig. S2a. A similar count of monocytes was observed in Tibetans between PHD2 heterozygous and wild-type, is shown in Fig. S2a. Reduced secretion of pro-inflammatory cytokines by PHD2^D4E;C127S monocytes than PHD2^WT. The CD14⁺ monocytes isolated from PBMCs were exposed to 1% oxygen for 24 hrs. CBA assay was used to assess supernatants for the levels of (d) IL6 and (e) IL1β, to compare between PHD2^D4E;C127S and PHD2^WT Tibetans. Unpaired t-test was used for comparison between genotypes, ***p<0.001 and **p<0.01. Levels of other cytokines as well as comparison of values amongst homozygous, heterozygous and wild type are mentioned in Fig. S3.