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. 2021 May 27;12:653088. doi: 10.3389/fimmu.2021.653088

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Copyright © 2021 Li, Frei, Labrada, Rong, Liang, Samojlik, Sun, Barash, Keselowsky, Bayer and Stabler

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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iTregs Induced by TGF-β1 PLGA Microparticles are Functionally Suppressive. Freshly isolated natural Tregs (nTregs), or iTregs generated by either TGF-β1 PLGA MPs or soluble TGF-β1 were mixed with CellTrace labeled CD4⁺Foxp3⁻T responder cells and stimulated with anti-CD3 in the presence of syngeneic APCs. Five different Tregs: Tconv ratios were tested (1:16, 1:8, 1:4, 1:2, and 1:1). (A) Representative responder cell proliferation histograms with the highest Treg dose (1:1 Treg : Tconv ratio) compared to control with no suppression. (B) Summary of the suppression of CD4⁺ responder T cell proliferation, as characterized by the percentage of dividing population normalized to the control with no suppression. N = 3, n = 10. n.s., not significant via two-way ANOVA test. (C) Non-linear suppression modeling of iTreg suppression of a representative test, where R^2 is the goodness-of-fit and IC50 is the half-maximal inhibitory concentration (inset).