Figure 7.

Representative Histological Images of Islet Graft Retrieved in Allogeneic Islet Transplantation. Representative H&E staining, Masson’s Trichrome staining, and immunohistochemistry staining for insulin (white), CD3 (green), Foxp3 (red), and DAPI (blue) on tissue from non-rejecting islet graft with TGF-β1 PLGA MPs (A–C), non-rejecting allogeneic islet graft-only (D–F), rejected allogeneic islet grafts (G–I), and rejected islet grafts with TGF-β1 PLGA MPs (J–L). Scale bars = 100 µm. Quantification of (M) intra-islet CD3⁺ cell infiltration for both engrafted and rejected allogeneic islet grafts, normalized to the total area of DAPI. (N) Quantification of intra-islet CD3⁺ cells, extra-islet CD3⁺ cells and global Foxp3⁺ cells normalized to the total area of DAPI were summarized for long-term engrafts recipients. t-test was performed between engrafted versus rejected islets, or TGF-β1 PLGA MPs treated and islet-only groups. n=5. ****p < 0.0001, *p < 0.05 and n.s., not significant.