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Characterization of iPSCs after gene editing
(A) Immunostaining of pluripotency markers (Nanog and TRA-1-60) in parental and APOE-/- iPSC lines.
(B) In vitro differentiation of parental and APOE-/- iPSC lines into cells of all three germ layers. Cells were immunostained for Sox17 (endoderm), Brachyury (mesoderm), Nestin (ectoderm), and DAPI (nucleus). Scale bar: 100 μm.
