Characterization of cerebral organoids at different time points
(A and B) Representative images of the ventricular zone (VZ)-like structure by Tuj1, Sox2 and Pax6 staining at Week 4 of differentiation.
(C and D) Representative images of Ctip2-positive (deep cortical layer marker) and Satb2-positive (superficial cortical layer marker) neurons at Week 4 and 12, respectively. This figure is adapted from Figure 1 in Zhao et al. (2020) (Zhao et al., 2020).
(E) Representative images of Tbr2-positive (intermediate progenitor marker) and Nkx2.1-positive (ventral region marker) cells. Figure reprinted with permission from Zhao et al. 2020.