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Guide RNA sequence for cloning in pX459 plasmid
| Guide RNA | gRNA sequence + adapter |
|---|---|
| gRNA2 F | CACCgAGCTGCGCCAGCAGACCGAG |
| gRNA2 R | aaacCTCGGTCTGCTGGCGCAGCTc |
| gRNA5 F | CACCgGGCCAAGGTGGAGCAAGCGG |
| gRNA5 R | aaacCCGCTTGCTCCACCTTGGCCc |
| gRNA8 F | CACCgACAGTGTCTGCACCCAGCGC |
| gRNA8 R | aaacGCGCTGGGTGCAGACACTGTc |
