Table 3.
Antibodies for immunofluorescence staining
| Antibody | Dilution | Supplier |
|---|---|---|
| Primary antibodies | ||
| Tuj1 (immunostaining) | 1:1000 | Sigma, T2200 |
| Tuj1 (immunostaining) | 1:1000 | Abcam, ab78078 |
| Sox2 (immunostaining) | 1:300 | Abcam, ab97959 |
| Ctip2 (immunostaining) | 1:100 | Abcam, ab18465 |
| Satb2 (immunostaining) | 1:100 | Abcam, ab34735 |
| GFAP (immunostaining) | 1:300 | Millipore, MAB360 |
| Pax6 (immunostaining) | 1:300 | Biolegend, 901302 |
| Tbr2 (immunostaining) | 1:300 | Abcam, ab23345 |
| Nkx2.1 (immunostaining) | 1:300 | Thermo, MA5-13961 |
| S100β (immunostaining) | 1:300 | Abcam, ab52642 |
| Nanog (immunostaining) | 1:300 | Cell Signaling, 4903S |
| TRA-1-60 (immunostaining) | 1:300 | Abcam, ab16288 |
| Nestin (immunostaining) | 1:300 | Abcam, ab18102 |
| Sox17 (immunostaining) | 1:300 | Abcam, ab84990 |
| Brachyury (immunostaining) | 1:300 | R&D Systems, AF2085 |
| ApoE (western blotting) | 1:1000 | Millipore, AB947 |
| Actin (western blotting) | 1:5000 | Sigma, A2228 |
| Secondary antibodies | ||
| Alexa Fluor donkey anti-rabbit 488, 594 | 1:500 | Invitrogen: A32790, A32754 |
| Alexa Fluor donkey anti-mouse 488, 594 | 1:500 | Invitrogen: A32766, A32744 |
| Alexa Fluor donkey anti-rat 488, 594 | 1:500 | Invitrogen: A21208, A21209 |
| Alexa Fluor donkey anti-goat 488 | 1:500 | Invitrogen: A-11055 |
| IRDye® 800CW Donkey anti-Goat IgG Secondary Antibody | 1:5000 | LI-COR: 926-32214 |
| IRDye® 680RD Donkey anti-Mouse IgG Secondary Antibody | 1:5000 | LI-COR: 926-68072 |