| Component | Amount |
|---|---|
| Plasmid | 1 μg |
| FastDigest BbsI | 1 μL (10 U/μL) |
| FastAP | 1 μL (1 U/μL) |
| 10× FastDigest Buffer | 2 μL |
| ddH2O | Supplement to 20 μL |
| Total | 20 μL |
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