| Component | Amount |
|---|---|
| Oligo 1 (gRNA F, 100 μM) | 1 μL |
| Oligo 2 (gRNA R, 100 μM) | 1 μL |
| 10× T4 Ligation Buffer | 1 μL |
| T4 PNK | 0.5 μL |
| ddH2O | 6.5 μL |
| Total | 10 μL |
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