| Component | Amount |
|---|---|
| BbsI digested plasmid from Step 2 | 50 ng |
| Phosphorylated and annealed oligo duplex from step 3 (1:200 dilution) | 1 μL |
| 2× Quick Ligation Buffer | 5 μL |
| Quick Ligase | 1 μL |
| ddH2O | Supplement to 10 μL |
| Total | 10 μL |
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