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. 2021 Jun 5;2(2):100571. doi: 10.1016/j.xpro.2021.100571
Component Amount
Ligation reaction from Step 4 10 μL
10× Plasmid Safe Buffer 1.5 μL
10 mM ATP 1.5 μL
Exonuclease 1 μL (10,000 U/mL)
ddH2O 1 μL
Total 15 μL