| Component | Amount |
|---|---|
| Template genomic DNA | 1.0 μL |
| APOE PCR Forward and Reverse primers | 2.5 μL |
| Phusion® DNA polymerase | 0.3 μL |
| 5× Phusion® HF buffer | 4.0 μL |
| 10 mM dNTP | 0.4 μL |
| DMSO (optional) | 1.0 μL |
| ddH2O | 10.8 μL |
| Total Vol. (μL) | 20 μL |
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