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. 2021 Jun 5;2(2):100571. doi: 10.1016/j.xpro.2021.100571
PCR Cycling Conditions
Steps Temperature Time Cycles
Initial denaturation 98°C 5 min 1
Denaturation 98°C 5 s 30
Annealing 66.6°C 10 s
Extension 72°C 30 s
Final extension 72°C 2 min 1
Hold 4°C