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. 2021 Jun 5;2(2):100571. doi: 10.1016/j.xpro.2021.100571

EB formation medium

Reagent Final Concentration Volume
Cerebral organoid basal medium 1 n/a 40 mL
Cerebral organoid supplement A n/a 10 mL
Total n/a 50 mL

Complete EB formation medium can be stored at 4°C for 1 week.