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. 2021 Jun 5;2(2):100571. doi: 10.1016/j.xpro.2021.100571

Expansion medium

Reagent Final Concentration Volume
Cerebral organoid basal medium 2 n/a 24.25 mL
Cerebral organoid supplement C n/a 0.25 mL
Cerebral organoid supplement D n/a 0.5 mL
Total n/a 25 mL

Complete Expansion medium can be stored at 4°C for 1 week.