Neuron medium for late stage cerebral organoid culture

Reagent	Final Concentration	Volume
DMEM/F-12	n/a	472 mL
Neurobasal	n/a	472 mL
B27 supplement with vitamin A (50×)	0.5×	10 mL
N2 supplement (100×)	0.5×	5 mL
Glutamax (100×)	1×	10 mL
sodium pyruvate mixture (100×)	1×	10 mL
Ascorbic acid (50 mg/mL stock)	200 ng/mL	4 μL
NEAA (100×)	1×	10 mL
dbCAMP (5 mg/mL stock)	500 ng/mL	100 μL
Penicillin-Streptomycin	1×	10 mL
BDNF (50  μg/mL stock)	10 ng/mL	200 μL
GDNF (50  μg/mL stock)	10 ng/mL	200 μL
Total		~ 1000 mL

Neuron medium can be stored at 4°C for 2 weeks. Add BDNF and GDNF right before use.