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. 2021 Jun 5;2(2):100571. doi: 10.1016/j.xpro.2021.100571

Blocking buffer for staining

Reagent Final Concentration Amount
Donkey serum 4% 4 mL
BSA 2% 2 g
Glycine 1 M 7.5 g
PBS n/a 96 mL
Total n/a ~ 100 mL

Blocking buffer can be stored at 4°C for 1 week. Aliquot can be made and stored in −20°C for 1 year.