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. Author manuscript; available in PMC: 2021 Jun 10.
Published in final edited form as: Cell Rep. 2020 Oct 6;33(1):108224. doi: 10.1016/j.celrep.2020.108224

Figure 2. A yeast model faithfully recapitulates APOE4-associated endocytic defects.

Figure 2.

(A) The estradiol-inducible promoter system used in the APOE yeast model. (B) Fluorescence microscopy of the mNeonGreen-tagged Vps21p. (C) Mean fluorescent signal from mNeonGreen-tagged Vps21p and Ypt53p quantified by flow cytometry, 10,000 cells per sample. (D) A schematic of the GSS invertase assay adapted. (E) Glucose signal from invertase assays of the indicated APOE-expressing strains and a TDP43-expressing strain. One-way ANOVA followed by Tukey’s multiple comparisons test (**** p-value<0.0001). Mean ± SD for n=3 independent colonies. See also Figure S3.