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. 2021 Jun 8;218(8):e20210542. doi: 10.1084/jem.20210542

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© 2021 Gratuze et al.

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Figure 2. — Altered microglia, either depleted, repopulated, or TREM2 KO, is associated with increased neuritic dystrophy around plaques, which correlates with NP-tau pathology. (A and B) Confocal analysis of Iba1⁺ microglia (red) surrounding X34⁺ plaques (blue) in ipsi- (A) and contralateral (B) cortices of 5XFAD mice. (C and D) Quantification of the number of microglia surrounding plaques in ipsi- (C) and contralateral (D) cortices. (E and F) Confocal images of BACE1 (red) around X34⁺ plaques (blue) in ipsi- (E) and contralateral (F) cortices of 5XFAD mice. (G and H) Quantification of percentage BACE1⁺ volume within 15 µm of plaques in ipsi- (G) and contralateral (H) cortices. (I) Representative image of costain for X34 (blue), AT8 (green), BACE1 (red), and Iba1 (white). (J) Relationship between costained Iba1⁺ microglia (x axis), BACE1 dystrophic neurites (y axis), and seeded AT8⁺ NP-tau surrounding X34⁺ plaques in the ipsilateral cortex. Each dot represents an individual mouse, dot color indicates the different groups, and dot size corresponds to percentage of AT8⁺ staining around plaques (Fig. 1). Scale bars, 50 µm. Data are presented as mean ± SEM. Significance was determined using ordinary one-way ANOVA followed by a Tukey's post hoc test (C and D) or Welch’s and Brown–Forsythe ANOVA test (G and H). *, P < 0.05; ***, P < 0.001; ****, P < 0.0001.