Figure 1.

A murine iPSC vaccine prevents tumor formation in vivo

(A) Diagram showing vaccine preparation consists of sorting murine iPSCs for pluripotency, irradiation, resuspension in adjuvant solution, and subcutaneous injection in the flank. Mice were randomized into different treatment groups and were vaccinated with the C + I vaccine, irradiated iPSCs alone, CpG alone, or PBS for 4 weeks.

(B) Vaccination of mice with the C + I vaccine resulted in a complete rejection of the cancer cells in six out of eight mice by day 49 and overall reductions in tumor size (n = 7–8 per group; representative images).

(C) Quantification of the tumor volume over time, with values being expressed as means ± SEM (n = 7–8, ^∗p < 0.05, ^∗∗p < 0.001, ^∗∗∗∗p < 0.0001, Tukey's multiple comparison test). Experiments were repeated three times to a total number of seven to eight mice per group.